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Determination of wash resistance
Washing procedure
Net samples (100 sq.cm) are individually introduced into 1 L screw-capped containing 0.5 L deionized water, with 2 g/L soap/detergent “ECE” (pH 10-11) added just before and fully dissolved. Beakers are immediately introduced into a water bath at 30 °C and shaken for 10 minutes at 200 rpm. Then, sample will be removed and rinsed twice for 10 minutes in clean deionized water in the same shaking conditions as stated above. Once washed, net samples are hung to dry overnight at room temperature. Between washings, nets are wrapped in aluminium foil and held in the dark in a climatic chamber at a constant 30 °C
Experimental design
Two net samples will be washed, in parallel at, 0, 5, 10, 15 and 20 times (one wash per day) then tested under the WHO cone test to asses KD effect and mortality. If nets fell below the cut off criteria using the WHO cone test (>80% mortality and/or > 95% KD), efficacy of nets washed 20 times will be estimate the tunnel test (WHO procedure) .
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